Solid Phase Extraction (SPE) and Flash Chromatography -- Applied Separations

Solid Phase Extraction Cartridges

Applied Separations offers a variety of Spe-ed (SPE) accessories, including medical grade polypropylene cartridges. The polypropylene cartridges are available in sizes ranging from 1mL to 60mL, MiniSpe-ed (Sep-Pak^TM type), and large reservoir cartridges (LRC). These cartridges can be purchased either empty or with frits.

Separate frits of assorted materials are also available in order to meet individual extraction needs. Syringe plungers are sold separately for manual extraction. These accessories, used separately or in combination, result in customized extraction columns for use in all solid phase extraction procedures.

Cartridge Types

	Standard Cartridges Standard extraction columns are straight walled syringe barrel tubes designed for fast, easy, and exact sample preparation. These cartridges are available in 6 different sizes ranging from 1mL to 60mL

	Large Reservoir Cartridges (LRC) The wide opening of the 500 mg (the nominal amount of sorbent) large reservoir cartridges (LRC) allow for sample or rinse volumes greater than that allowed by the standard cartridge. The flared shape accommodates 15mL of sample without using additional reservoirs or adaptors.

	Flangeless/Rimless Today’s fully automated SPE processing systems require an SPE cartridge configuration that has been specially adapted to fit these instruments. Applied Separations’ Flangeless cartridges do not have the flange, or tab that is found at the top of Applied Separations’ Standard SPE cartridge. The flangeless design allows for closer packing in specialized automated SPE systems, such as the Gilson® SPE215. This format will accommodate 96 x 1mL cartridges, or 48 x 3mL cartridges in the standard 96-well footprint.

	Mini-Spe-ed MiniSpe-ed cartridges contain the wide variety of sorbents found in standard Spe-ed cartridges. The MiniSpe-ed configuration can be easily used in manual syringe operations or on a vacuum manifold.

	Spe-ed 96 Make your solid phase extraction (SPE) sample preparation easier, faster, and more economical with Spe-ed 96 Microtiter Plates from Applied Separations filled with any sorbant to meet your needs.

	Glass Cartridges When a high level of purity is absolutely necessary for your most critical determination, glass cartridges from Applied Separations fit the bill. Not only can you get them empty, but we have devised a method to fill them with a packing/sorbent of your choice and send them to you, ready to use.

Filled Cartridges

Many of our standard silica-based sorbents are packed and available off the shelf for shipping the next business day in the following configurations. For additional packings, frits/filters, cartridge size, type, and sorbent weight, please contact us.

Sorbent packed in standard 1mL, 3mL, 6mL, or 12mL polypropylene cartridge syringe barrels with standard 20µ hydrophobic polyethelyne frit
Sorbent			100mg 1mL	200mg 3mL	500mg 3mL	500mg 6mL	1000mg 6mL	1000mg 12mL
	C18	Octadecyl/14	2001	2002	2003	2006	2007	2009
	C18	Octadecyl/18	12001	12002	12003	12006	12007	12009
	C18	Octadecyl/22	12101	12102	12103	12106	12107	12109
	C18	Octadecyl/OH (not endcapped)	12201	12202	12203	12206	12207	12209
	C8	Octyl	2011	2012	2013	2016	2017	2019
	PH	Phenyl	2081	2082	2083	2086	2087	2089
	CH	Cyclohexyl	2071	2072	2073	2076	2077	2079
	C4	Butyl	2021	2022	2023	2026	2027	2029
	C2	Ethyl	2061	2062	2063	2066	2067	2069
	C1	Methyl	2051	2052	2053	2056	2057	2059
	CNe	Cyanopropyl	2221	2222	2223	2226	2227	2229
	CN	Cyanopropyl (not endcapped)	2201	2202	2203	2206	2207	2209
	SI	Silica Gel	2101	2102	2103	2106	2107	2109
	FLO	Florisil	2111	2112	2113	2116	2117	2119
	ALN	Alumina (Neutral)	2121	2122	2123	2126	2127	2129
	ALA	Alumina (Acidic)	2131	2132	2133	2136	2137	2139
	ALB	Alumina (Basic)	2141	2142	2143	2146	2147	2149
	DIO	Diol	2151	2152	2153	2156	2157	2159
	COOH	Carboxylic Acid	2311	2312	2313	2316	2317	2319
	SCX	Benzenesulfonic Acid	2321	2322	2323	2326	2327	2329
	NH2	Aminiopropyl	2211	2212	2213	2216	2217	2219
	PSA	Pri/Sec Amine	2241	2242	2243	2246	2247	2249
	DEA	Diethylamino	2331	2332	2333	2336	2337	2339
	N+	Quaternary Amino	2301	2302	2303	2306	2307	2309
Cartridges Per Box			100	50	50	30	30	20

Sorbent packed in standard 6mL, 12mL, 35mL, or 60mL polypropylene cartridge syringe barrels with standard 20µ hydrophobic polyethelyne frit
Sorbent			2g 6mL	2g 12mL	2g 35mL	3g 12mL	3g 35mL	5g 35mL	10g 60mL	20g 60mL
	C18	Octadecyl/14	2008	5001	5002	5003	5004	5005	5007	5008
	C18	Octadecyl/18	12008	15001	15002	15003	15004	15005	15007	15008
	C18	Octadecyl/22	12108	15101	15102	15103	15104	15105	15107	15108
	C18	Octadecyl/OH (not endcapped)	12208	15201	15202	15203	15204	15205	15207	15208
	C8	Octyl	2018	5011	5012	5013	5014	5015	5017	5018
	PH	Phenyl	2088	5081	5082	5083	5084	5085	5087	5088
	CH	Cyclohexyl	2078	5071	5072	5073	5074	5075	5077	5078
	C4	Butyl	2028	5021	5022	5023	5024	5025	5027	5028
	C2	Ethyl	2068	5061	5062	5063	5064	5065	5067	5068
	C1	Methyl	2058	5051	5052	5053	5054	5055	5057	5058
	CNe	Cyanopropyl	2228	5221	5222	5223	5224	5225	5227	5228
	CN	Cyanopropyl (not endcapped)	2208	5201	5202	5203	5204	5205	5207	5208
	SI	Silica Gel	2108	5101	5102	5103	5104	5105	5107	5108
	FLO	Florisil	2118	5111	5112	5113	5114	5115	5117	5118
	ALN	Alumina (Neutral)	2128	5121	5122	5123	5124	5125	5127	5128
	ALA	Alumina (Acidic)	2138	5131	5132	5133	5134	5135	5137	5138
	ALB	Alumina (Basic)	2148	5141	5142	5143	5144	5145	5147	5148
	DIO	Diol	2158	5151	5152	5153	5154	5155	5157	5158
	COOH	Carboxylic Acid	2318	5311	5312	5313	5314	5315	5317	5318
	SCX	Benzenesulfonic Acid	2328	5321	5322	5323	5324	5325	5327	5328
	NH2	Aminiopropyl	2218	5211	5212	5213	5214	5215	5217	5218
	PSA	Pri/Sec Amine	2248	5241	5242	5243	5244	5245	5247	5248
	DEA	Diethylamino	2338	5331	5332	5333	5334	5335	5337	5338
	N+	Quaternary Amino	2308	5301	5302	5303	5304	5305	5307	5308
Cartridges Per Box			30	20	20	20	20	20	12	12

Applied Separations also offers contract packing of sorbents

Proprietary sorbents that need to be packed in an SPE cartridge
Special vials, containers, cartridges or housings that need to be packed with sorbent
An unusual or non-standard quantity of sorbent to be packed in an SPE cartridge

Ordering info:
Click here to download our complete Standard Cartridge list.

Request a quote on the our cartridges or any other SPE products from Applied Separations.

Applications
We have compiled a comprehensive list of SPE and Flash chromatography applications available for free. Click here to view the list of the the applications you can put to use immediately in your lab.